comparison of excretory secretory antigens of ascaris lumbricoldes and toxocara cati 2nd. stage larvae with biochemical methodes

purification of parasitic antigens is a major activity in immunoparasitology because of its application in immunodiagnosis, vaccination analysis of immunopathology, preparation of monocolomal antibody and finding out the cross reactive antigens versus specific antigens of a parasite. in this survey f2 ultrafiltration excretory secretory antigens of ascaris lumbricoides and toxocara cati were separated by gel chromatography on sephacryl s-200 into several antigenic and non antigenic fractions. the e/s antigens of this nematode were analyzed by using soudium dodecyl sulfate polyacryl amide gel electrophoresis (sds page analysis of ascaris lumbricoides and toxocara cati revealed a minimum of 12 kd to 130 kd distinct with brilliant coomassie blue staining bands respectively.